Epitope Mapping

Epitopes are specific sites on antigen molecules that are recognized by antibodies. Structural studies showed that around 15 amino acid residues are often associated with each epitope (Johne B (1998). Mol Biotechnol 9: 6). Epitope mapping on the biosensor can be used to characterize both antigens and monoclonal antibodies. Compared to the labeling requirements involving EIA or RIA, using biosensor technology brings many advantages as fast response, simple performance of experiments and low sample consumption. Typical epitope mapping experiments on the biosensor involve immobilizing the primary antibody on surface, then capturing the antigen and testing if a secondary antibody is capable of binding to the antigen. Using this method it is possible to screen a variety of monoclonal antibodies specific to different epitopes presented on antigen.